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当前位置: 首页 > 产品中心 > Transfection > Calbiotech/Mouse/Rat ACTH Ultra Sensitive lumELISA/AC562T-100
商品详细Calbiotech/Mouse/Rat ACTH Ultra Sensitive lumELISA/AC562T-100
Calbiotech/Mouse/Rat ACTH Ultra Sensitive lumELISA/AC562T-100
Calbiotech/Mouse/Rat ACTH Ultra Sensitive lumELISA/AC562T-100
商品编号: AC562T-100
品牌: Calbiotech
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 转染试剂
公司分类: Transfection
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PRINCIPLE OF THE TESTThe CBIACTH Immunoassay is a two-site lumELISA (Chemiluninescence Enzyme-Linked ImmunoSorbent Assay) for the measurement of the biologically active 39 amino acid chain of ACTH. A goat polyclonal antibody to ACTH, purified by affinity chromatography, and a mouse monoclonal antibody to ACTH are specific for well-defined regions on the ACTH molecule. One antibody is prepared to bind only the C-terminal ACTH 34-39 and this antibody is biotinylated. The other antibody is prepared to bind only the mid-region and N-terminal ACTH 1-24 and this antibody is labeled with horseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidin-coated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components. Upon the addition of the luminol substrate, the enzyme activity in the enzyme-bound fraction is directly proportional to the concentration of the ACTH in the sample. A standard curve is prepared relating light unit (RLU) to the concentration of the ACTH. Concentrations of ACTH present in the controls and samples are determined directly from this curve.

AC562T-100-R4-RCType : PDF

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Calbiotech公用:清洗,停止,TMB,PBS排序方式产品名称+/-结果1-5共5  10  15  30  60  150 产品名称产品价格PBS缓冲液SKU: PBMF130$ 50.00产品详情停止解决方案SKU: CC604SS$ 10.00产品详情测试SKU: 测试$ 1.00产品详情TMB基板SKU: CC608TM$ 20.00产品详情洗涤缓冲液SKU: CC606WC$ 10.00产品详情