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Calbiotech/Mouse/Rat HSV-1 IgG ELISA/96 Tests (12x8 breakable strip wells)/H1029G-100
| Catalog number | H1029G-100 |
| Product type | ELISA |
| Quantity | 96 Tests (12x8 breakable strip wells) |
| Sample volume | 10 µl/well |
| Species | Mouse or Rat |
| Application | The Calbiotech Mouse HSV-1 IgG ELISA test system is an enzyme linked immunosorbent assay (ELISA) used for the detection of IgG class antibodies to HSV-1 in mouse serum or plasma. |
| Principle | Diluted serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. |
| Storage and Stability | Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1. Markoulatos P; Fountoucidou P; Marinakis G; Krikelis V; Spyrou N; Vamvakopoulos N; Moncany ML. Clear detection and typing of herpes simplex virus types 1 and 2 by an indirect ELISA assay: comparison with three different combined methods--capture ELISA, restriction enzymes, and polymerase chain reaction. J Clin Lab Anal 1997; 11(3):146-53. 2. Nahmias AJ, Roizman B. Infection with herpes-simplex viruses 1 and 2. II. N Engl J Med. 1973 289(14):719-25. 3. McKendall RR. Comparative neurovirulence and latency of HSV1 and HSV2 following footpad inoculation in mice. J Med Virol. 1980;5(1):25-32 4. Dawson GJ, Mowshowitz SL, Cohen R, Elizan TS. Herpes simplex virus persistence in mouse neuroblastoma (C 1300) cell cultures: role of interferon. J Neural Transm. 1984; 59(4):309-17. 6. Dawson DW; Fish DI; Shackleton P The accuracy and clinical interpretation of serum ferritin assays. Clin Lab Haematol 1992; 14(1):47-52. |
H1029G-100-R4-RCType : PDF
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