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当前位置: 首页 > 产品中心 > Virus_purification_kit > Calbiotech/Mouse/Rat Testosterone, Total ELISA/TE187S-100
商品详细Calbiotech/Mouse/Rat Testosterone, Total ELISA/TE187S-100
Calbiotech/Mouse/Rat Testosterone, Total ELISA/TE187S-100
Calbiotech/Mouse/Rat Testosterone, Total ELISA/TE187S-100
商品编号: TE187S-100
品牌: Calbiotech
市场价: ¥11000.00
美元价: 5500.00
产地: 美国(厂家直采)
公司:
产品分类: 病毒纯化试剂盒
公司分类: Virus_purification_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Catalog numberTE187S-100
Product typeELISA
Quantity96 Tests (12x8 breakable strip wells)
Standard range0.1-18 ng/ml
Analytical Sensitivity0.1 ng/ml
Sample volume25 µl/well
SpeciesMouse or Rat
ApplicationThe Calbiotech Mouse/Rat Testosterone ELISA is for the quantitative determination of Testosterone concentration in mouse/rat serum or plasma.
Principle
The Calbiotech Mouse/Rat Testosterone ELISA is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25ml of Testosterone standards, controls, patient samples, 100 ml Testosterone-HRP conjugate reagent and 50ml rabbit anti-Testosterone reagent at room temperature 60 minutes. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.
Storage and StabilityProduct should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.
PrecautionsFor research use only. Not for use in diagnostic procedures.
References

1. Chen, A., Bookstein, J.J., Meldrum, D.R., Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertil. Steril., 1991; 55: 1202-1203.

2. Granoff, A.B. and Abraham, G.E., Peripheral and adrenal venous levels of steroids in a patient with virilizing adrenal adenoma. Obstet. Gynecol., 1979; 53:111-115.

3. Bricaire, C., Raynaud, A., Benotmane, A., et al., Selective venous catheterization in the evaluation of hyperandrogenism. J. Endocrinol Invest., 1991; 14: 949-956.

4. Heinonen, P.K., Androgen production by epithelial ovarian tumours in post-menopausal women. Maturitas, 1991; 13: 117-117-122

5. Tietz, N.W. ed., Clinical Guide to Laboratory Tests, 3rd Edition, W.B. Saunders, Co., Philadelphia, 1995: 578-580.

6. USA Center for Disease Control/National Institute of Health Manual, “Biosafety in Microbiological and Biomedical Laboratories"”84

7. ICN Guide to Endocrine Testing. Diagnostic Division, ICN Biomedicals, Inc. pp. 2:33-35; 3:4-6.

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品牌介绍
Calbiotech公用:清洗,停止,TMB,PBS排序方式产品名称+/-结果1-5共5  10  15  30  60  150 产品名称产品价格PBS缓冲液SKU: PBMF130$ 50.00产品详情停止解决方案SKU: CC604SS$ 10.00产品详情测试SKU: 测试$ 1.00产品详情TMB基板SKU: CC608TM$ 20.00产品详情洗涤缓冲液SKU: CC606WC$ 10.00产品详情